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Cryogel™ SepA

Syringe with protein A plug. System for cell separation with no chemical elution.
- excellent recovery
- high viability
- simple operation

For more information please click Product Specification.
SepA description[1].pdf

Preferred references (see Publications);
A Kumar, FM Plieva, IYu Galaev, B Mattiasson (2003) Affinity fractionation of lymphocytes using supermacroporous monolithic cryogel. J. Immunol. Methods 283, 185-194.
A Kumar, A Rodriguez-Cabaelero, FM Plieva, IYu Galaev, KS Nandukumar, M Kamihira, R Holmdahl, A Orfao, B Mattiasson (2005) Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture. J. Mol. Recognit. 18, 84-93
MB Dainiak, A Kumar, IYu Galaev, B Mattiasson (2006) Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel. Proc. Natl. Acad. Sci. USA 103, 849-854.

Cryogel™ HTS E

96-minicolumn plates with epoxy activated plugs for separation and analysis of cells and nanoparticles

- integrated separation and analysis
- simple operation
- excellent screening tool

For more information please click Product Specification.
HTS E description[1].pdf

Preferred references (see Publications)
IYu Galaev, MB Dainiak, FM Plieva, R Hatti-Kaul, B Mattiasson (2005) High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format. J. Chromatogr. A, 1065, 169-175.
A Hanora, F Bernaudat, FM Plieva, MB Dainiak, L Bulow, I Yu Galaev, B Mattiasson (2005) Screening of peptide affinity chromatography in 96-well plate format. J. Chromatogr. A, 1087, 38-44.
MB Dainiak, IYu Galaev, B Mattiasson (2007) Macroporous monolithic hydorgels in a 96-minicolumn plate format for cell surface-analysis and integrated binding/quantification of cells. Enzyme Microb. Technol., 40, 688-695.
S Deraz, FM Plieva, IYu Galaev, E Nordberg Karlsson, B Mattiasson (2007) Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands. Enyme. Microb. Technol ., 40, 786-793.


Cryogel™ E

Epoxy activated dry plugs that fit normal 5 mL columns.
Plugs for multipurpose cell and nanoparticle separation
- excellent recovery
- simple operation
- your choice of ligand
- wide range of use

For more information click Product Specification.
Cryogel E description[1].pdf


Preferred references (See Publications);
MB Dainiak, FM Plieva, IYu Galaev, R Hatti-Kaul, B Mattiasson (2005) Cell chromatography. Separation of different microbial cells using IMAC supermacroporous monolithic columns. Biotechnol. Progr. 21, 644-649.
W Noppe, FM Plieva, K Vanhoorelbeke, H Deckmyn, M Tuncel, A Tuncel, IYu Galaev, B Mattiasson (2007) Macroporous gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds. J. Biotechnol., 131, 293-299.
FM Plieva, E De Seta, IYu Galaev, B Mattiasson (2008) Macroporous elastic polyacrylamide monolith columns: processing under compression and scale-up. Sep Pur Technol. /in press, available online 27 March 2008)
MB Dainiak, IYu Galaev, B Mattiasson (2006) Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells. J. Chromatogr. A 1123, 145-150.
MB Dainiak, A Kumar, FM Plieva, IYu Galaev, B Mattiasson (2004) Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels. J. Chromatogr. A, 1045, 93-98.
MB Dainiak, A Kumar, IYu Galaev, B Mattiasson (2006) Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel. Proc. Natl. Acad. Sci. USA 103, 849-854.


More information contact info@protista.se or call +46 (0) 42 82910.



 

Protista International AB  |  P.O. Box 86  |  SE-267 22 Bjuv, Sweden  |  Phone +46 42 829 10  |  Fax +46 42 833 01  |  info@protista.se